She obtained her B.Eng in Chemical Engineering and Ph.D in Bioengineering from the National University of Singapore in 2001 and 2008 respectively. This paper describes the fabrication of complex networks of microchannel via modularized stereolithography 3D printing. It amused me to know about how the graphics and programs were squeezed in 16KB ROM cartridge and executed by an 8bit processor with just 3 internal resistors (Accumulator, X, Y) a 256 Bytes of RAM (127 Bytes of RAM for Variables & 127 Bytes shared with Television Adaptor Video memory) Akshaya Srinivasan, Yi-Chin Toh*, Human Pluripotent Stem Cell-derived Neural Crest Cells for Tissue Regeneration and Disease Modeling, 2019, Frontiers in Molecular Neuroscience, 12, https://doi.org/10.3389/fnmol.2019.00039, This paper reviews the various in vitro strategies that have been used to derive NCCs from hPSCs and specify NCCs into cranial, trunk and vagal subpopulations and their derivatives. We use cookies to help provide and enhance our service and tailor content and ads. and Yu, H., 2013. To make microfluidic pumps more accessible to the scientific community, researchers from the Singapore University of Technology and Design (SUTD) Soft Fluidics Lab developed a highly-customizable, 3D-printed peristaltic pump kit, where users from around the world can download the design files, 3D-print and assemble their do-it-yourself (DIY) peristaltic pump (refer to image). Michinao received his B.S. This is a collaborative effort with Dr Thomas Willett @ Waterloo University demonstrating the utility of 3D printed soybean-based scaffolds for bone tissue engineering application. Akshaya Srinivasan, Nelson Teo, Poon Kei Jun, Priya Tiwari, Akhilandeshwari Ravichandran, Wen Feng, Swee Hin Teoh, Thiam Chye Lim, Yi-Chin Toh*, Comparative Craniofacial Bone Regeneration Capacities of Mesenchymal Stem Cells Derived from Human Neural Crest Stem Cells and Bone Marrow, 2021, ACS Biomaterial Science and Research, 7, 1, 207221.
He currently sits on the editorial boards of more than 20 international journals. Biomicrofluidics, 2020, 14, (5), DOI: 10.1063/5.0012185. and Yu, H., 2013. This paper reports a double nuclear-staining technique in combination with confocal image processing to visualize and quantify cell viability in microfluidic 3D tissue constructs. Xianzhu Zhang, Dandan Cai, Feifei Zhou, Jie Yu, Xinyu Wu, Dongsheng Yu, Yiwei Zou, Yi Hong, Chunhui Yuan, Yishan Chen, Zongyou Pan, Varitsara Bunpetch, Heng Sun, Chengrui An, Yi-Chin Toh, Hongwei Ouyang, Shufang Zhang, Targeting downstream subcellular YAP activity as a function of matrix stiness with Verteporn-encapsulated chitosan microsphere attenuates osteoarthritis, 2020,Biomaterials, 232: 119724, Terry Ching, Yi-Chin Toh, Michinao Hashimoto, Fabrication of Complex 3D Fluidic Networks via Modularized Stereolithography, 2019, Advanced Engineering Materials, 1901109, https://doi.org/10.1002/adem.201901109. Anene-Nzelu, C.G., Peh, K.Y., Fraiszudeen, A., Kuan, Y.H., Ng, S.H.,Toh, Y.C. Tasnim, F., Toh, Y.C., Qu, Y., Li, H., Phan, D., Narmada, B.C., Ananthanarayanan, A., Mittal, N., Meng, R.Q. #semiconductor Dysregulation of the human microbiome has been linked to various disease states, which has galvanized the efforts to modulate human health through microbiomes.
Louis Jun Ye Ong, Liang Zhu, Gabriel Jenn Sern Tan,Yi-Chin Toh*, Quantitative Image-Based Cell Viability (QuantICV) Assay for Microfluidic 3D Tissue Culture Applications, 2020,Micromachines, 11 (7): 10.3390/mi11070669. and Yu, H., 2008. Currently, his groups research directions include 3D printing and bioprinting for microfluidic devices, low-cost instrumentation, flexible devices, and 3D food printing. Besides her research interests, she loves keeping abreast of the latest trends in innovations and technologies, reading, and learning new languages.
We also discovered that substrate stiffness modulated the differentiation of NCSCs to eMSCs via a Rho-ROCK dependent pathway involving CD44-mediated inhibition of PDGFR signaling. By applying the modularized approach, 2D and 3D networks of microchannels with high complexity (i.e., multiple branching networks) and small crosssectional dimensions (w=75m and h=100m) are fabricated in photoresins and hydrogels. She did her post-doctoral training at the Massachusetts Institute of Technology in 2008 under Professor Joel Voldmans guidance. Candidate under a joint program between SUTD and the National University of Singapore (NUS) under the guidance of Michinao Hashimoto (SUTD) and Yi-Chin Toh (NUS). Prof Lim has co-founded six startups which are commercializing technologies developed in his lab. Her research interests include developing microfluidic-based cell culture models specifically for gastro and hepatology studies and their applications towards metabolic health and diseases. Youve made so much possible over the past half-century. The pump is powered and controlled by Arduino, an open-source electronics platform. This study is a comprehensive evaluation of the effects of various topographical cues on arterial- or venous-specific EC phenotype and function. degrees in Chemistry and Biochemistry/Biophysics from Oregon State University (2003), and Ph.D. degree from Harvard University (2009), followed by postdoctoral training at Massachusetts Institute of Technology and Childrens Hospital Boston. Scientific Reports,7. Cellular responses to a nanofibrous environment, 2006,NanoToday, 1(3): 34-43. Download : Download high-res image (220KB)Download : Download full-size image. He has been also awarded with theZwick Science Awardfor his studies on Mechanical Testing, and best PhD thesis at the University of Barcelona for studies in the use of biopolymers inMicroelectronicsandBiomedicine. }. It was very interesting and I had learned a lot about old ROM Programming, Sprits design, 6507 CPU chip, etc. Dendrimer hydrazides asmultivalent transient inter cellular linkers, Biomaterials, 2008, 29 (27): 3693-3702, Ong, S.M., Zhang, C.,Toh, Y.C., Kim, S.H., Foo, H.L., Tan, C.H., van Noort, D., Park, S. and Yu, H., 2008. To make microfluidic pumps more accessible to the scientific community, Singapore University of Technology and Design (SUTD) researchers have developed a highly-customizable and Arduino-powered peristaltic pump kit. Development of poly (lactic-co-glycolic acid)-collagen scaffolds for tissue engineering. Most craniofacial bones are derived from the ectodermal germ layer via neural crest stem cells, which are distinct from mesoderm-derived long bones. Prof Lims research interests are interdisciplinary and include human disease mechanobiology, microfluidic biomedical technologies for human disease diagnosis and precision medicine and soft wearable technologies for healthcare applications. Seep Arora, Christine Cheung, Evelyn Yim*, Yi-Chin Toh*, Topography elicits distinct phenotypes and functions in human primary and stem cell derived endothelial cells, 2020, Biomaterials, 234, 119747. Yi-Chin Toh is a Future Fellow and Associate Professor at the Queensland University of Technology. Hsih Yin Tan and Yi-Chin Toh*, What can microfluidics do for microbiome research? Copyright 2022 Elsevier B.V. or its licensors or contributors. His research focuses on novel 3D printing methods to fabricate microfluidics, organs-on-a-chip, and biomimetic vasculature. | Insanely Complex, Abbott Launches 5-Minute Virus Test for Use Almost Anywhere, How a Computer Chip Can Smell without a Nose. Cellular responses to a nanofibrous environment, A Configurable 3D Microenvironment in a Micro-fluidic Channel forPrimary Hepatocytes Culture, Application of polyelectrolyte complex coacervation to improve the seeding efficiency of bone marrow stromal cells in 3D scaffolds, Decolourisation of azo dyes by white-rot fungi (WRF) isolated in Singapore. This resulted in the formation of a reproducible and highlyorganized NE tissue. A novel 3D mammalian cell perfusion-culture system in microfluidic channels. Her research interest is in engineering multi-scale tissue models to mimic complex biological interactions during human development and diseases, as well as translating them into scalable platforms for disease modeling and drug testing applications. Herein, we developed a cost-effective kit to build a micro-peristaltic pump (~ 50 USD) consisting of 3D-printed and off-the-shelf components. Toh, Y.C., Ng, S., Khong, Y.M., Samper, V. and Yu, H., 2005. After his Ph.D, Javier moved to MIT, where he developed the Micro-Masonry, a technology to assemble artificial organs and recognized as breakthrough in tissue engineering (CNET).
Lor Huai Chong, Celine Ng, Huan Li, Edmund Feng Tian, Abhishek Ananthanarayanan, Michael McMillian, Yi-Chin Toh, Hepatic Bioactivation of Skin-Sensitizing Drugs to Immunogenic Reactive Metabolites, 2019,ACS Omega,4, 9, 13902-13912. *,A 3D Printed Microfluidic Perfusion Device for Multicellular Spheroid Cultures, 2017,Biofabrication, Ong, L.J.Y., Chong, L.H., Jin, L., Singh, P.K., Lee, P.S., Yu, H., Ananthanarayanan, A., Leo, H.L.,Toh, Y.C.
Rapid customization of the instrument would benefit users. Today my grandkids can explore the universe from a handheld computer. She is also an aspiring photographer. In this paper, we combine hPSC micropatterning and a temporallysequenced induction protocol to specify neuroepithelial (NE) cells in spatial juxtaposition to mesoendoderm cells. At the same time, it generated tremendous respect for all the design engineers who used to work in the dark ages of electronics before HDL and EDA. Javier regularly collaborates with some of the most important international media outlets. They developed a highly-customizable, 3D-printed peristaltic pump kit. This paper discusses how microfluidic technologies can value add to the different phases of human microbiome research as well as the unique challenges that microfluidics must overcome when working with microbiome-relevant biological materials. Published by Elsevier Ltd. https://doi.org/10.1016/j.ohx.2021.e00202. Jyothsna joined The Fermart Lab in 2016 as NUS-SUTD PhD fellow. 2D And 3D Combo Could Keep Moores Law Going. Yi-Chin Toh, Anju Raja, Hanry Yu, Danny van Noort, A 3D Microfluidic Model to Recapitulate Cancer Cell Migration and Invasion, 2018, Bioengineering, 5(2): 29, Live visualisation and tracking of breast cancer cells migrating across an ECM barrier in a microfluidic 3D tumor model, Srinivasan, A., Chang, S.Y., Zhang, S., Toh, W.S.,Toh, Y.C. We also discuss the potential applications of these human specific NCC platforms, including the use of iPSCs for disease modeling and the potential of NCCs for future regenerative applications, Seep Arora, Jing Ying Adele Lam, Christine Cheung, Evelyn KF Yim, Yi-Chin Toh*, Determination of critical shear stress for maturation of human pluripotent stem cell derived endothelial cells towards an arterial subtype, 2019, Biotechnology and Bioengineering, 116 (5), 1164-1175, https://doi.org/10.1002/bit.26910.
Prior to her research stint at NUS, she completed her Bachelors degree in Biotechnology from SRM University, Chennai. Mechanical compaction directly modulates the dynamics of bile canaliculi formation, 2013,Integrative Biology, 5 (2): 390 400. display: none !important; Seep Arora, Akshaya Srinivasan, Chak Ming Leung,Yi-Chin Toh*, Bio-mimicking shear stress environments for enhancing mesenchymal stem cell differentiation, 2020, Current Stem Cell Research & Therapy, 15(5):414-427. This paper describes a modular microfluidic platform that enables a user-intuitive and flexible way to configure multi-organ perfusion culture systems. Seep, Evelyn Yim, Yi-Chin Toh*, Environmental specification of pluripotent stem cell derived endothelial cells towards arterial and venous subtypes,2019, Frontiers in Bioengineering and Biotechnologyhttps://doi.org/10.3389/fbioe.2019.00143. This is the biggest circuit I have simulated till today without HDL.
Here, we designed a 3D printable micro-peristaltic pump kit as a cost-effective (~50 USD) alternative to current commercial pumps (>1000 USD). To demonstrate the forward compatibility, we developed a flowrate calibration tool that was readily interfaced with the developed pump system.
A Practical Guide to Microfluidic Perfusion Culture of Adherent Mammalian Cells. By continuing you agree to the use of cookies. Happy 50th Birthday 4004 microchip! A method for human teratogen detection by geometrically confined cell differentiation and migration. He is also an avid proponent for open-source, low-cost development of low-cost scientific instruments. Thanks to Mr. Gustavo for designing such a wonderful course. The peristaltic pump kit exhibited good repeatability in flow rates and functioned for 7 days without any noticeable issues in performance. This is achieved by dispensing a fast-curing flexible silicone resin on various substrates to form microchannels. In 2014 he was awarded the worlds most outstanding young researcher in materials science by the Bayer Foundation. Application of polyelectrolyte complex coacervation to improve the seeding efficiency of bone marrow stromal cells in 3D scaffolds. Ong, L.J.Y., Islam, A.B., DasGupta, R., Iyer, N.G., Leo., H.L.,Toh, Y.C. At The Fermart Lab, she is working towards building in vitro lab-on-a-chip platforms to study cell migration and cancer metastasis cascade. You can contribute your articles on emerging fields toPioneering Minds. This paper demonstrated that substratestiffness mediated mechanical cues can directly modulate the development of ectodermal MSCs(eMSCs) from a precursor human neural crest stem cell (NCSC) population. *,A pump-free microfluidic 3D perfusion platform for the efficient differentiation of human hepatocyte-like cells, 2017,Bioengineering & Biotechnology. This paper reports a liver-immune coculture array that can mimic liver-mediated bioactivation of parent drugs into reactive metabolites and their subsequent activation of immune cells. With an overarching research theme of microfluidics, the group works on various cross-disciplinary themes in biomedical engineering, digital fabrication, and food engineering. 2007,Materials Science and Engineering: C,27(2): 285292.
What Would Happen To Our Bodies After Death In Space? Lor Huai Chong, Huan Li, Isaac Wetzel, Hansang Cho, Yi-Chin Toh*, A liver-immune coculture array for predicting systemic drug-induced skin sensitization, 2018, Lab on a Chip 18: 3239. Being Restless and curious, I invested my time and solved it. The controlled presentation of TGF-b1 to hepatocytes in a 3D microfludic cell culture system, 2009,Biomaterials, 2009, 30: 3847-3853, Zhang, S., Xia, L., Kang, C.H., Xiao, G., Ong, S.M.,Toh, Y.C., Leo, H.L., van Noort, D., Kan, S.H., Tang, H.H. Different endothelial cell subtypes often exhibit similar morphological response to topographical cues, however, they can demonstrate different phenotypic and functional responses. nine
Microfluidic systems for lab-on-a-chip (LoC) and organs-on-a-chip (OoC) applications require precise fluid flow control.
Bob Noyces integrated circuits and those 4KB took us to the moon. Microfabricated silicon nitride membranes for hepatocyte sandwich culture, 2008,Biomaterials, 29 (29): 3993-4002, Zhao, D., Ong, S.M., Yue, Z., Jiang, Z.,Toh, Y.C., Khan, M., Shi, J., Tan, C.H., Chen, J.P. and Yu, H., 2008. Integrating sensitive quantification of hepatic metabolic functions by capillary electrophoresis with laser-induced fluorescence detection, 2008,Analyst, 133: 326-330, Wen, F., Chang, S.,Toh, Y.C., Arooz, T., Zhuo, L., Teoh, S.H. and Yu, H., 2013. Samper, D. van Noort, D.W. Hutmacher and H.Yu. Leveraging on being smallSingapores strategy to catalyze integrative innovations, 2011,Lab on a Chip, 11: 1853-1855(Invited article), Toh, Y.C. The pumps exhibited good repeatability in flowrates and functioned inside a cell incubator (at 37C and 95 % humidity) for seven days without noticeable issues in the performance. By evaluating NE tissue structural dysmorphia in the micropatterned NE model, we can successfully model gene- and drug-induced neurodevelopmental defects.
Please enter your username or email address.
The pump is designed in the form of a kit, allowing end-users to customize the setup. He is an elected Fellow of the American Institute for Medical and Biological Engineering (AIMBE), International Academy of Medical and Biological Engineering (IAMBE), Academy of Engineering, Singapore and the Singapore National Academy of Science. ScienceDirect is a registered trademark of Elsevier B.V. ScienceDirect is a registered trademark of Elsevier B.V. Highly-customizable 3D-printed peristaltic pump kit.
A gel-free 3D microfluidic cell culture system, 2008,Biomaterials, 29(22): 3237-3244, Toh, Y.C., Zhang, J., Khong, Y.M., Du, Y., Sun, W. and Yu, H., 2008. Commercially available peristaltic pumps for microfluidics are usually bulky, expensive, and not customizable. Sahni, G.,Yuan, J.andToh, Y.C. Decolourisation of azo dyes by white-rot fungi (WRF) isolated in Singapore. *, Substrate stiffness modulates the multipotency of neural crest derived human ectomesenchymal stem cells via CD44 mediated PDGFR signaling, 2018,Biomaterials167:153-167. Development of dual-compartment perfusionbioreactor for serial coculture of hepatocytes and stellate cells in poly (lactic-co-glycolic acid)-collagen scaffolds,Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2008, 87(1): 154-162. Our generation is blessed enough to leverage the advantage of Automation tools.
and Yu, H., 2007.
2016,Molecular Pharmaceutics,13(6), pp.1947-1957. Enzyme and Microbial Technology, 2003, 33(5): 569-575. The assembled pumps offered a flowrate of 0.02 ~ 727.3L/min, and the smallest pump assembled with this kit was 205028mm. We demonstrated fabricating two variants of pumps with different sizes and operating flowrates using the developed kit. Featured in Global Medical Discovery(ISSN 1929-8536),Featured in Global Medical Discovery (ISSN 1929-8536), Jan 2013, Toh, Y.C., Blagovic, K., Yu, H. and Voldman, J., 2011. Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates.
He currently sits on the editorial boards of more than 20 international journals. Biomicrofluidics, 2020, 14, (5), DOI: 10.1063/5.0012185. and Yu, H., 2013. This paper reports a double nuclear-staining technique in combination with confocal image processing to visualize and quantify cell viability in microfluidic 3D tissue constructs. Xianzhu Zhang, Dandan Cai, Feifei Zhou, Jie Yu, Xinyu Wu, Dongsheng Yu, Yiwei Zou, Yi Hong, Chunhui Yuan, Yishan Chen, Zongyou Pan, Varitsara Bunpetch, Heng Sun, Chengrui An, Yi-Chin Toh, Hongwei Ouyang, Shufang Zhang, Targeting downstream subcellular YAP activity as a function of matrix stiness with Verteporn-encapsulated chitosan microsphere attenuates osteoarthritis, 2020,Biomaterials, 232: 119724, Terry Ching, Yi-Chin Toh, Michinao Hashimoto, Fabrication of Complex 3D Fluidic Networks via Modularized Stereolithography, 2019, Advanced Engineering Materials, 1901109, https://doi.org/10.1002/adem.201901109. Anene-Nzelu, C.G., Peh, K.Y., Fraiszudeen, A., Kuan, Y.H., Ng, S.H.,Toh, Y.C. Tasnim, F., Toh, Y.C., Qu, Y., Li, H., Phan, D., Narmada, B.C., Ananthanarayanan, A., Mittal, N., Meng, R.Q. #semiconductor Dysregulation of the human microbiome has been linked to various disease states, which has galvanized the efforts to modulate human health through microbiomes.
Louis Jun Ye Ong, Liang Zhu, Gabriel Jenn Sern Tan,Yi-Chin Toh*, Quantitative Image-Based Cell Viability (QuantICV) Assay for Microfluidic 3D Tissue Culture Applications, 2020,Micromachines, 11 (7): 10.3390/mi11070669. and Yu, H., 2008. Currently, his groups research directions include 3D printing and bioprinting for microfluidic devices, low-cost instrumentation, flexible devices, and 3D food printing. Besides her research interests, she loves keeping abreast of the latest trends in innovations and technologies, reading, and learning new languages.
We also discovered that substrate stiffness modulated the differentiation of NCSCs to eMSCs via a Rho-ROCK dependent pathway involving CD44-mediated inhibition of PDGFR signaling. By applying the modularized approach, 2D and 3D networks of microchannels with high complexity (i.e., multiple branching networks) and small crosssectional dimensions (w=75m and h=100m) are fabricated in photoresins and hydrogels. She did her post-doctoral training at the Massachusetts Institute of Technology in 2008 under Professor Joel Voldmans guidance. Candidate under a joint program between SUTD and the National University of Singapore (NUS) under the guidance of Michinao Hashimoto (SUTD) and Yi-Chin Toh (NUS). Prof Lim has co-founded six startups which are commercializing technologies developed in his lab. Her research interests include developing microfluidic-based cell culture models specifically for gastro and hepatology studies and their applications towards metabolic health and diseases. Youve made so much possible over the past half-century. The pump is powered and controlled by Arduino, an open-source electronics platform. This study is a comprehensive evaluation of the effects of various topographical cues on arterial- or venous-specific EC phenotype and function. degrees in Chemistry and Biochemistry/Biophysics from Oregon State University (2003), and Ph.D. degree from Harvard University (2009), followed by postdoctoral training at Massachusetts Institute of Technology and Childrens Hospital Boston. Scientific Reports,7. Cellular responses to a nanofibrous environment, 2006,NanoToday, 1(3): 34-43. Download : Download high-res image (220KB)Download : Download full-size image. He has been also awarded with theZwick Science Awardfor his studies on Mechanical Testing, and best PhD thesis at the University of Barcelona for studies in the use of biopolymers inMicroelectronicsandBiomedicine. }. It was very interesting and I had learned a lot about old ROM Programming, Sprits design, 6507 CPU chip, etc. Dendrimer hydrazides asmultivalent transient inter cellular linkers, Biomaterials, 2008, 29 (27): 3693-3702, Ong, S.M., Zhang, C.,Toh, Y.C., Kim, S.H., Foo, H.L., Tan, C.H., van Noort, D., Park, S. and Yu, H., 2008. To make microfluidic pumps more accessible to the scientific community, Singapore University of Technology and Design (SUTD) researchers have developed a highly-customizable and Arduino-powered peristaltic pump kit. Development of poly (lactic-co-glycolic acid)-collagen scaffolds for tissue engineering. Most craniofacial bones are derived from the ectodermal germ layer via neural crest stem cells, which are distinct from mesoderm-derived long bones. Prof Lims research interests are interdisciplinary and include human disease mechanobiology, microfluidic biomedical technologies for human disease diagnosis and precision medicine and soft wearable technologies for healthcare applications. Seep Arora, Christine Cheung, Evelyn Yim*, Yi-Chin Toh*, Topography elicits distinct phenotypes and functions in human primary and stem cell derived endothelial cells, 2020, Biomaterials, 234, 119747. Yi-Chin Toh is a Future Fellow and Associate Professor at the Queensland University of Technology. Hsih Yin Tan and Yi-Chin Toh*, What can microfluidics do for microbiome research? Copyright 2022 Elsevier B.V. or its licensors or contributors. His research focuses on novel 3D printing methods to fabricate microfluidics, organs-on-a-chip, and biomimetic vasculature. | Insanely Complex, Abbott Launches 5-Minute Virus Test for Use Almost Anywhere, How a Computer Chip Can Smell without a Nose. Cellular responses to a nanofibrous environment, A Configurable 3D Microenvironment in a Micro-fluidic Channel forPrimary Hepatocytes Culture, Application of polyelectrolyte complex coacervation to improve the seeding efficiency of bone marrow stromal cells in 3D scaffolds, Decolourisation of azo dyes by white-rot fungi (WRF) isolated in Singapore. This resulted in the formation of a reproducible and highlyorganized NE tissue. A novel 3D mammalian cell perfusion-culture system in microfluidic channels. Her research interest is in engineering multi-scale tissue models to mimic complex biological interactions during human development and diseases, as well as translating them into scalable platforms for disease modeling and drug testing applications. Herein, we developed a cost-effective kit to build a micro-peristaltic pump (~ 50 USD) consisting of 3D-printed and off-the-shelf components. Toh, Y.C., Ng, S., Khong, Y.M., Samper, V. and Yu, H., 2005. After his Ph.D, Javier moved to MIT, where he developed the Micro-Masonry, a technology to assemble artificial organs and recognized as breakthrough in tissue engineering (CNET).
Lor Huai Chong, Celine Ng, Huan Li, Edmund Feng Tian, Abhishek Ananthanarayanan, Michael McMillian, Yi-Chin Toh, Hepatic Bioactivation of Skin-Sensitizing Drugs to Immunogenic Reactive Metabolites, 2019,ACS Omega,4, 9, 13902-13912. *,A 3D Printed Microfluidic Perfusion Device for Multicellular Spheroid Cultures, 2017,Biofabrication, Ong, L.J.Y., Chong, L.H., Jin, L., Singh, P.K., Lee, P.S., Yu, H., Ananthanarayanan, A., Leo, H.L.,Toh, Y.C.
Rapid customization of the instrument would benefit users. Today my grandkids can explore the universe from a handheld computer. She is also an aspiring photographer. In this paper, we combine hPSC micropatterning and a temporallysequenced induction protocol to specify neuroepithelial (NE) cells in spatial juxtaposition to mesoendoderm cells. At the same time, it generated tremendous respect for all the design engineers who used to work in the dark ages of electronics before HDL and EDA. Javier regularly collaborates with some of the most important international media outlets. They developed a highly-customizable, 3D-printed peristaltic pump kit. This paper discusses how microfluidic technologies can value add to the different phases of human microbiome research as well as the unique challenges that microfluidics must overcome when working with microbiome-relevant biological materials. Published by Elsevier Ltd. https://doi.org/10.1016/j.ohx.2021.e00202. Jyothsna joined The Fermart Lab in 2016 as NUS-SUTD PhD fellow. 2D And 3D Combo Could Keep Moores Law Going. Yi-Chin Toh, Anju Raja, Hanry Yu, Danny van Noort, A 3D Microfluidic Model to Recapitulate Cancer Cell Migration and Invasion, 2018, Bioengineering, 5(2): 29, Live visualisation and tracking of breast cancer cells migrating across an ECM barrier in a microfluidic 3D tumor model, Srinivasan, A., Chang, S.Y., Zhang, S., Toh, W.S.,Toh, Y.C. We also discuss the potential applications of these human specific NCC platforms, including the use of iPSCs for disease modeling and the potential of NCCs for future regenerative applications, Seep Arora, Jing Ying Adele Lam, Christine Cheung, Evelyn KF Yim, Yi-Chin Toh*, Determination of critical shear stress for maturation of human pluripotent stem cell derived endothelial cells towards an arterial subtype, 2019, Biotechnology and Bioengineering, 116 (5), 1164-1175, https://doi.org/10.1002/bit.26910.
Prior to her research stint at NUS, she completed her Bachelors degree in Biotechnology from SRM University, Chennai. Mechanical compaction directly modulates the dynamics of bile canaliculi formation, 2013,Integrative Biology, 5 (2): 390 400. display: none !important; Seep Arora, Akshaya Srinivasan, Chak Ming Leung,Yi-Chin Toh*, Bio-mimicking shear stress environments for enhancing mesenchymal stem cell differentiation, 2020, Current Stem Cell Research & Therapy, 15(5):414-427. This paper describes a modular microfluidic platform that enables a user-intuitive and flexible way to configure multi-organ perfusion culture systems. Seep, Evelyn Yim, Yi-Chin Toh*, Environmental specification of pluripotent stem cell derived endothelial cells towards arterial and venous subtypes,2019, Frontiers in Bioengineering and Biotechnologyhttps://doi.org/10.3389/fbioe.2019.00143. This is the biggest circuit I have simulated till today without HDL.
Here, we designed a 3D printable micro-peristaltic pump kit as a cost-effective (~50 USD) alternative to current commercial pumps (>1000 USD). To demonstrate the forward compatibility, we developed a flowrate calibration tool that was readily interfaced with the developed pump system.
A Practical Guide to Microfluidic Perfusion Culture of Adherent Mammalian Cells. By continuing you agree to the use of cookies. Happy 50th Birthday 4004 microchip! A method for human teratogen detection by geometrically confined cell differentiation and migration. He is also an avid proponent for open-source, low-cost development of low-cost scientific instruments. Thanks to Mr. Gustavo for designing such a wonderful course. The peristaltic pump kit exhibited good repeatability in flow rates and functioned for 7 days without any noticeable issues in performance. This is achieved by dispensing a fast-curing flexible silicone resin on various substrates to form microchannels. In 2014 he was awarded the worlds most outstanding young researcher in materials science by the Bayer Foundation. Application of polyelectrolyte complex coacervation to improve the seeding efficiency of bone marrow stromal cells in 3D scaffolds. Ong, L.J.Y., Islam, A.B., DasGupta, R., Iyer, N.G., Leo., H.L.,Toh, Y.C. At The Fermart Lab, she is working towards building in vitro lab-on-a-chip platforms to study cell migration and cancer metastasis cascade. You can contribute your articles on emerging fields toPioneering Minds. This paper demonstrated that substratestiffness mediated mechanical cues can directly modulate the development of ectodermal MSCs(eMSCs) from a precursor human neural crest stem cell (NCSC) population. *,A pump-free microfluidic 3D perfusion platform for the efficient differentiation of human hepatocyte-like cells, 2017,Bioengineering & Biotechnology. This paper reports a liver-immune coculture array that can mimic liver-mediated bioactivation of parent drugs into reactive metabolites and their subsequent activation of immune cells. With an overarching research theme of microfluidics, the group works on various cross-disciplinary themes in biomedical engineering, digital fabrication, and food engineering. 2007,Materials Science and Engineering: C,27(2): 285292.
What Would Happen To Our Bodies After Death In Space? Lor Huai Chong, Huan Li, Isaac Wetzel, Hansang Cho, Yi-Chin Toh*, A liver-immune coculture array for predicting systemic drug-induced skin sensitization, 2018, Lab on a Chip 18: 3239. Being Restless and curious, I invested my time and solved it. The controlled presentation of TGF-b1 to hepatocytes in a 3D microfludic cell culture system, 2009,Biomaterials, 2009, 30: 3847-3853, Zhang, S., Xia, L., Kang, C.H., Xiao, G., Ong, S.M.,Toh, Y.C., Leo, H.L., van Noort, D., Kan, S.H., Tang, H.H. Different endothelial cell subtypes often exhibit similar morphological response to topographical cues, however, they can demonstrate different phenotypic and functional responses. nine
Microfluidic systems for lab-on-a-chip (LoC) and organs-on-a-chip (OoC) applications require precise fluid flow control. Bob Noyces integrated circuits and those 4KB took us to the moon. Microfabricated silicon nitride membranes for hepatocyte sandwich culture, 2008,Biomaterials, 29 (29): 3993-4002, Zhao, D., Ong, S.M., Yue, Z., Jiang, Z.,Toh, Y.C., Khan, M., Shi, J., Tan, C.H., Chen, J.P. and Yu, H., 2008. Integrating sensitive quantification of hepatic metabolic functions by capillary electrophoresis with laser-induced fluorescence detection, 2008,Analyst, 133: 326-330, Wen, F., Chang, S.,Toh, Y.C., Arooz, T., Zhuo, L., Teoh, S.H. and Yu, H., 2013. Samper, D. van Noort, D.W. Hutmacher and H.Yu. Leveraging on being smallSingapores strategy to catalyze integrative innovations, 2011,Lab on a Chip, 11: 1853-1855(Invited article), Toh, Y.C. The pumps exhibited good repeatability in flowrates and functioned inside a cell incubator (at 37C and 95 % humidity) for seven days without noticeable issues in the performance. By evaluating NE tissue structural dysmorphia in the micropatterned NE model, we can successfully model gene- and drug-induced neurodevelopmental defects.
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The pump is designed in the form of a kit, allowing end-users to customize the setup. He is an elected Fellow of the American Institute for Medical and Biological Engineering (AIMBE), International Academy of Medical and Biological Engineering (IAMBE), Academy of Engineering, Singapore and the Singapore National Academy of Science. ScienceDirect is a registered trademark of Elsevier B.V. ScienceDirect is a registered trademark of Elsevier B.V. Highly-customizable 3D-printed peristaltic pump kit.
A gel-free 3D microfluidic cell culture system, 2008,Biomaterials, 29(22): 3237-3244, Toh, Y.C., Zhang, J., Khong, Y.M., Du, Y., Sun, W. and Yu, H., 2008. Commercially available peristaltic pumps for microfluidics are usually bulky, expensive, and not customizable. Sahni, G.,Yuan, J.andToh, Y.C. Decolourisation of azo dyes by white-rot fungi (WRF) isolated in Singapore. *, Substrate stiffness modulates the multipotency of neural crest derived human ectomesenchymal stem cells via CD44 mediated PDGFR signaling, 2018,Biomaterials167:153-167. Development of dual-compartment perfusionbioreactor for serial coculture of hepatocytes and stellate cells in poly (lactic-co-glycolic acid)-collagen scaffolds,Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2008, 87(1): 154-162. Our generation is blessed enough to leverage the advantage of Automation tools.
and Yu, H., 2007. 2016,Molecular Pharmaceutics,13(6), pp.1947-1957. Enzyme and Microbial Technology, 2003, 33(5): 569-575. The assembled pumps offered a flowrate of 0.02 ~ 727.3L/min, and the smallest pump assembled with this kit was 205028mm. We demonstrated fabricating two variants of pumps with different sizes and operating flowrates using the developed kit. Featured in Global Medical Discovery(ISSN 1929-8536),Featured in Global Medical Discovery (ISSN 1929-8536), Jan 2013, Toh, Y.C., Blagovic, K., Yu, H. and Voldman, J., 2011. Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates.